Colibri Cytometry

If you don't put in the legwork of testing the mixes for your panel, expect to run into problems.

How to prepare and store antibody master mixes stably.

Running large panels consistently is all about error prevention.

Why do we lose GFP and other fluorescent proteins when we fix and permeabilize cells? How can we stop this?

Does overnight staining help for antigen-specific TCR detection with tetramers? Not so much.

What's the best type of fluorophore to use for targets that are in the nucleus of the cell?

Our samples, more often than not, contain autofluorescence (AF) that varies not only in intensity but also in aspect.

Today I'm going to share my tips for successfully running lots of samples on the Aurora without having to babysit the machine.

Let’s start by looking at the autofluorescence extraction methods available to us, their advantages and drawbacks.

When unmixing, we have the option to use either an internal negative population or a completely independent "Universal Negative".

Let's take a step back and look at how autofluorescence is handled in conventional flow.

Backgating is a simple, useful technique in flow cytometry that can help you figure out where your cells are, and thus optimize your gating

Today let’s look at something I think you shouldn’t be doing with AF extraction in spectral flow cytometry.

There are three key ways in which AutoSpill differs from traditional compensation: Data usage, AF handling and Refinement.

In this series on autofluorescence (AF), I’ll post some musings on how AF affects flow cytometry data and approaches for dealing with it.

You've designed your big flow panel--a great accomplishment! But wait, there's more work to do before you use it.

Reduce the cost of your panels by focusing on the most expensive reagents.

What's the best way to stain for human CCR7?

What are the benefits and drawbacks of staining at higher temperatures?