Colibri Cytometry

Our samples, more often than not, contain autofluorescence (AF) that varies not only in intensity but also in aspect.

Let’s start by looking at the autofluorescence extraction methods available to us, their advantages and drawbacks.

Let's take a step back and look at how autofluorescence is handled in conventional flow.

Today let’s look at something I think you shouldn’t be doing with AF extraction in spectral flow cytometry.

In this series on autofluorescence (AF), I’ll post some musings on how AF affects flow cytometry data and approaches for dealing with it.