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![Dealing with multiple autofluorescences](https://static.wixstatic.com/media/2bf391_96a3d530ab95488197542c02d3bb5610~mv2.png/v1/fill/w_514,h_386,fp_0.50_0.50,q_95,enc_auto/2bf391_96a3d530ab95488197542c02d3bb5610~mv2.webp)
Dec 2, 20248 min read
Dealing with multiple autofluorescences
Our samples, more often than not, contain autofluorescence (AF) that varies not only in intensity but also in aspect.
582 views4 comments
![](https://static.wixstatic.com/media/2bf391_0963cc35315644df97d342bd53502d59~mv2.jpg/v1/fill/w_250,h_250,fp_0.50_0.50,q_30,blur_30,enc_auto/2bf391_0963cc35315644df97d342bd53502d59~mv2.webp)
![Five ways to extract autofluorescence (and store it)](https://static.wixstatic.com/media/2bf391_0963cc35315644df97d342bd53502d59~mv2.jpg/v1/fill/w_514,h_386,fp_0.50_0.50,q_90,enc_auto/2bf391_0963cc35315644df97d342bd53502d59~mv2.webp)
Nov 18, 202412 min read
Five ways to extract autofluorescence (and store it)
Let’s start by looking at the autofluorescence extraction methods available to us, their advantages and drawbacks.
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![Autofluorescence in conventional flow cytometry](https://static.wixstatic.com/media/2bf391_ae75e1096e3145c79a8f04baa56b3fc5~mv2.png/v1/fill/w_514,h_386,fp_0.50_0.50,q_95,enc_auto/2bf391_ae75e1096e3145c79a8f04baa56b3fc5~mv2.webp)
Nov 4, 20247 min read
Autofluorescence in conventional flow cytometry
Let's take a step back and look at how autofluorescence is handled in conventional flow.
816 views2 comments
![Autofluorescence: what not to do](https://static.wixstatic.com/media/2bf391_387f1fb83a3442408d08acc56ba1f940~mv2.png/v1/fill/w_514,h_386,fp_0.50_0.50,q_95,enc_auto/2bf391_387f1fb83a3442408d08acc56ba1f940~mv2.webp)
Oct 21, 20244 min read
Autofluorescence: what not to do
Today let’s look at something I think you shouldn’t be doing with AF extraction in spectral flow cytometry.
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![Thoughts on autofluorescence](https://static.wixstatic.com/media/2bf391_0cc02e940a724e69982bce825c61980c~mv2.png/v1/fill/w_514,h_386,fp_0.50_0.50,q_95,enc_auto/2bf391_0cc02e940a724e69982bce825c61980c~mv2.webp)
Oct 1, 20244 min read
Thoughts on autofluorescence
In this series on autofluorescence (AF), I’ll post some musings on how AF affects flow cytometry data and approaches for dealing with it.
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