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Oct 28, 20243 min read
Backgating: What is it and why will it help?
Backgating is a simple, useful technique in flow cytometry that can help you figure out where your cells are, and thus optimize your gating
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Jul 10, 20241 min read
Flowcytoscript update
The Liston-Dooley lab's flowcytoscript for simplified cytometry data analysis has been updated.
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Jun 3, 20244 min read
What's that cluster? Part II
If you're interested in this problem, you may wish to get in touch with Ryan Brinkman of Dotmatics regarding the SOULCAP project.
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May 27, 20244 min read
Data Analysis: Comparing Dimensionality Reductions
In this post , I compared a few dimensionality reduction approaches for visualizing flow cytometry data. I plotted the flow cytometry...
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May 13, 20245 min read
Data analysis: Scaling your data for dimensionality reduction
Today we're talking about scales. Very boring, very important.
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Apr 22, 20244 min read
Data Analysis: EmbedSOM's party trick
Tired of trying to waiting for tSNE to run on large data sets? What if we could run any dimensionality reduction approach, but in a...
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Apr 8, 20245 min read
What's that cluster?
Today I'm going to talk about a feature in flowcytoscript--an R-based workflow for flow-cytometry analysis from the Liston Lab--that...
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Mar 25, 202410 min read
Data Analysis: Dimensionality Reduction
In this series, we're going to look at dimensionality reduction algorithms for use in the display and analysis of high parameter flow...
1,418 views6 comments
Dec 11, 20231 min read
Data analysis: Comparing tSNE plots
Most of the time when people generate tSNE plots of flow data, they're interested in comparing how a treatment or condition affects the...
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