Colibri Cytometry

Why do we lose GFP and other fluorescent proteins when we fix and permeabilize cells? How can we stop this?

Today I'm going to share my tips for successfully running lots of samples on the Aurora without having to babysit the machine.

An overview of protocols for measuring different modalities by flow cytometry

How do we deal with marker downregulation during intracellular cytokine staining?

When we conjugate an antibody to a fluorophore, we are performing a chemical reaction, creating a covalent bond between the fluorophore and

In this post , I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...

Intracellular cytokine staining can be challenging to get right. The cells need to be in great condition to withstand the stimulation...

Titration of antibodies means finding the optimal amount to use in order to maximize signal (specific binding) while minimizing noise...

Learn how to use AutoSpill to get practically perfect compensation every time.