Colibri Cytometry

Does overnight staining help for antigen-specific TCR detection with tetramers? Not so much.

What's the best type of fluorophore to use for targets that are in the nucleus of the cell?

What are the benefits and drawbacks of staining at higher temperatures?

How do we deal with marker downregulation during intracellular cytokine staining?

The idea? Combine overnight staining with RNA sequencing.

In this post , I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...

Watch this talk to learn how leaving your staining for longer can help you get better data for cheaper. Overcome issues of low...

Which fixative should I use? PFA? A kit? Maybe formalin and dish soap? Answer: It depends! Honestly, it depends a lot on what you're...

Today I'm going to discuss batch effects, both some thoughts about what causes them and how using overnight staining can help reduce...

Before we get started, it's worth pointing out that while overnight staining is new to flow cytometry, it is pretty commonly used in...

Probably the most frequent question I get about overnight staining is along the lines of "won't my cells die?" So today, I'm going to...

Today I'm starting a series of posts about how to use overnight staining to improve your flow cytometry. I developed this approach over...