Colibri Cytometry

Let’s start by looking at the autofluorescence extraction methods available to us, their advantages and drawbacks.

When unmixing, we have the option to use either an internal negative population or a completely independent "Universal Negative".

There are three key ways in which AutoSpill differs from traditional compensation: Data usage, AF handling and Refinement.

Cell-based controls are inherently multi-colour controls. Understanding this is key to getting good unmixing.

Why do we want multiple controls? The most common reason, I expect, is that you'll have a control that you aren't sure will work.

Today’s post is about how to get the best results with spectral unmixing. Poor quality unmixing is the most common problem I help people...

Learn how to use AutoSpill to get practically perfect compensation every time.