Colibri Cytometry

Cell-based controls are inherently multi-colour controls. Understanding this is key to getting good unmixing.

Why do we want multiple controls? The most common reason, I expect, is that you'll have a control that you aren't sure will work.

As much as possible, avoid combining multiple factors that cause tandem breakdown.

CellBlox: a blocking solution for working with NovaFluors

The issue: Interactions between Brilliant dyes on antibody conjugates.

Monocyte Block destroys your cells. When used intracellularly, Monocyte Block reduces transcription factor staining.

Let's discuss Fc block, which is what most people think of when we talk about "blocking" for flow cytometry.

The Liston-Dooley lab's flowcytoscript for simplified cytometry data analysis has been updated.

Today we're looking at the most basic of blocking reagents: generic protein.

In this series, I’m going to review blocking reagents and how to use them for best effect.

An overview of protocols for measuring different modalities by flow cytometry

If you're interested in this problem, you may wish to get in touch with Ryan Brinkman of Dotmatics regarding the SOULCAP project.

In this post , I compared a few dimensionality reduction approaches for visualizing flow cytometry data. I plotted the flow cytometry...

How do we deal with marker downregulation during intracellular cytokine staining?

Today we're talking about scales. Very boring, very important.

The idea? Combine overnight staining with RNA sequencing.

When we conjugate an antibody to a fluorophore, we are performing a chemical reaction, creating a covalent bond between the fluorophore and

Tired of trying to waiting for tSNE to run on large data sets? What if we could run any dimensionality reduction approach, but in a...