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Jul 294 min read
Blocking: Brilliant Dye interactions
The issue:
Interactions between Brilliant dyes on antibody conjugates.
797 views2 comments
Jul 223 min read
Blocking: Monocyte block
Monocyte Block destroys your cells.
When used intracellularly, Monocyte Block reduces transcription factor staining.
620 views3 comments
Jul 156 min read
Blocking: Fc block and related reagents
Let's discuss Fc block, which is what most people think of when we talk about "blocking" for flow cytometry.
1,748 views8 comments
Jul 101 min read
Flowcytoscript update
The Liston-Dooley lab's flowcytoscript for simplified cytometry data analysis has been updated.
153 views0 comments
Jul 82 min read
Blocking: the value of protein
Today we're looking at the most basic of blocking reagents: generic protein.
720 views2 comments
Jul 12 min read
Blocking: a cure for all your problems or a cause of all your problems?
In this series, I’m going to review blocking reagents and how to use them for best effect.
1,229 views2 comments
Jun 241 min read
Colibri resources
If you haven't looked beyond the blog, today is the day to do so. Colibri Cytometry provides other resources, which are highlighted in...
181 views0 comments
Jun 171 min read
How to do different types of flow
An overview of protocols for measuring different modalities by flow cytometry
898 views0 comments
Jun 34 min read
What's that cluster? Part II
If you're interested in this problem, you may wish to get in touch with Ryan Brinkman of Dotmatics regarding the SOULCAP project.
391 views3 comments
May 274 min read
Data Analysis: Comparing Dimensionality Reductions
In this post , I compared a few dimensionality reduction approaches for visualizing flow cytometry data. I plotted the flow cytometry...
587 views0 comments
May 203 min read
Cytokine staining: When to stain
How do we deal with marker downregulation during intracellular cytokine staining?
1,495 views0 comments
May 135 min read
Data analysis: Scaling your data for dimensionality reduction
Today we're talking about scales. Very boring, very important.
1,577 views0 comments
May 34 min read
Overnight staining for scRNA-Seq?
The idea? Combine overnight staining with RNA sequencing.
268 views3 comments
Apr 297 min read
How to conjugate your own antibodies
When we conjugate an antibody to a fluorophore, we are performing a chemical reaction, creating a covalent bond between the fluorophore and
1,008 views0 comments
Apr 224 min read
Data Analysis: EmbedSOM's party trick
Tired of trying to waiting for tSNE to run on large data sets? What if we could run any dimensionality reduction approach, but in a...
371 views0 comments
Apr 152 min read
Optimizing cytokine staining--fixatives
In this post , I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...
1,266 views2 comments
Apr 85 min read
What's that cluster?
Today I'm going to talk about a feature in flowcytoscript--an R-based workflow for flow-cytometry analysis from the Liston Lab--that...
595 views3 comments
Apr 12 min read
Optimizing cytokine staining--Ionomycin
Intracellular cytokine staining can be challenging to get right. The cells need to be in great condition to withstand the stimulation...
917 views0 comments
Mar 2510 min read
Data Analysis: Dimensionality Reduction
In this series, we're going to look at dimensionality reduction algorithms for use in the display and analysis of high parameter flow...
1,338 views6 comments
Mar 182 min read
Panel Design: Build a backbone
I suggest starting by designing what I call a backbone for your panel. This is just the basic gating markers that will allow you to...
781 views2 comments
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