Colibri Cytometry

In this series, we're going to look at dimensionality reduction algorithms for use in the display and analysis of high parameter flow...

I suggest starting by designing what I call a backbone for your panel. This is just the basic gating markers that will allow you to...

How to pick the best fluorophores to use in your flow panels.

Once we've decided which markers to include in our panel, the next step is to begin selecting the fluorophores that we want to use in the...

Spread is an inevitable consequence of multicolour fluorescence-based flow cytometry. When an excited fluorophore emits lower energy...

Today let's look at web-tools for understanding fluorophore signatures. These spectral viewer tools can be very useful for designing flow...

A flow panel is all about choices. Our choices with the markers we include and the signals we attach to them via antibodies create a...

Errors in flow data are all too common. The key to reducing errors lies in being able to recognize the symptoms and distinguish between...

Titration of antibodies means finding the optimal amount to use in order to maximize signal (specific binding) while minimizing noise...

Today's topic is benchmarking. This is probably particularly relevant for anyone who manages a shared resource lab with an Aurora. On the...

Improve your signal by using more antibodies, not more antibody. If you have a marker that doesn't stain well, sometimes you can improve...

Most of the time when people generate tSNE plots of flow data, they're interested in comparing how a treatment or condition affects the...

Tandems, tandems, tandems. We can't seem to avoid them in high parameter flow experiments--they're just too useful. How do we deal with...

Today’s post is about how to get the best results with spectral unmixing. Poor quality unmixing is the most common problem I help people...

Watch this talk to learn how leaving your staining for longer can help you get better data for cheaper. Overcome issues of low...

Which fixative should I use? PFA? A kit? Maybe formalin and dish soap? Answer: It depends! Honestly, it depends a lot on what you're...

Today I'm going to discuss batch effects, both some thoughts about what causes them and how using overnight staining can help reduce...

Before we get started, it's worth pointing out that while overnight staining is new to flow cytometry, it is pretty commonly used in...

Probably the most frequent question I get about overnight staining is along the lines of "won't my cells die?" So today, I'm going to...

Today I'm starting a series of posts about how to use overnight staining to improve your flow cytometry. I developed this approach over...