Spectrum viewer tools

Webtools for visualizing and comparing fluorophore spectra.

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• FluoroFinder

• Sony ID7000

• Cytek Cloud

• BioLegend Aurora

• BioLegend ID7000

• Bio-Rad

• BD Spectrum Viewer

• BD Research Cloud

• ThermoFisher

• Beckman Coulter

• Miltenyi Biotec

Antibody compatibility

• Epitope retention post-fixation (BioLegend). Note: this is mostly accurate for PFA/formalin fixation. Fixing with Fix/Perm reagents will retain many more epitopes.

• Antibody performance post-fix (Thermo)

• Antibody performance with Phosflow buffers (BD)

Learn to code

• RStudio

• Python

• Github

• Stack Overflow

• ChatGPT (really useful for learning)

• Bioconductor flowCore

• Swirl (learn R)

Other tools

• Human Cell Atlas

• ImmGen

• BD Interactive Cell Map

• BD Clone Comparison Tool

• BD Research Cloud

Other educational resources

• University of Chicago

• ChUG Podcast

• Bitesizebio

• ThermoFisher

• BioLegend

• BD webinars

• Applied Cytometry

• FlowStars

• FluoroFinder articles

• FlowJo articles

• FlowJo University

• FCS Express Academy

• OMIQ resources

• Cytek webinars

• Cytek SpectroLearn

• ISAC Learn

• OMIP_ISAC

Data analysis services

This isn't an endorsement of any of these services. If you're looking for analysis options for your high dimensional or high throughput data, these might provide solutions, at a cost.

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Note that the free coding ecosystem for flow cytometry data analysis is quite well developed in both R and Python.

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• Floreada.io (free)

• OMIQ

• TerraFlow

• Cytolytics

• Cytobank

• Tercen